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Figure 1. Limit of detection experiment for the ureA assay, tested against a tenfold dilution series of H. <t>suis</t> DNA. Cycle threshold (Ct) values are tabulated (above). Amplification plots of a 10-fold dilution series on H. suis DNA samples, containing 2.22 × 107 to 2.22 × 101 bacterial genome equivalents per reaction, are shown (below). The baseline threshold (horizontal red line) is set at 337800 to optimise signal-to-noise.
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Figure 1. Limit of detection experiment for the ureA assay, tested against a tenfold dilution series of H. <t>suis</t> DNA. Cycle threshold (Ct) values are tabulated (above). Amplification plots of a 10-fold dilution series on H. suis DNA samples, containing 2.22 × 107 to 2.22 × 101 bacterial genome equivalents per reaction, are shown (below). The baseline threshold (horizontal red line) is set at 337800 to optimise signal-to-noise.
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Figure 1. Limit of detection experiment for the ureA assay, tested against a tenfold dilution series of H. <t>suis</t> DNA. Cycle threshold (Ct) values are tabulated (above). Amplification plots of a 10-fold dilution series on H. suis DNA samples, containing 2.22 × 107 to 2.22 × 101 bacterial genome equivalents per reaction, are shown (below). The baseline threshold (horizontal red line) is set at 337800 to optimise signal-to-noise.
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Figure 1. Limit of detection experiment for the ureA assay, tested against a tenfold dilution series of H. suis DNA. Cycle threshold (Ct) values are tabulated (above). Amplification plots of a 10-fold dilution series on H. suis DNA samples, containing 2.22 × 107 to 2.22 × 101 bacterial genome equivalents per reaction, are shown (below). The baseline threshold (horizontal red line) is set at 337800 to optimise signal-to-noise.

Journal: International journal of molecular sciences

Article Title: Non- Helicobacter pylori Helicobacters , a Treatable Provocateur of Parkinson's Disease: Hypothesis, Evidence and Species Specificity.

doi: 10.3390/ijms252313123

Figure Lengend Snippet: Figure 1. Limit of detection experiment for the ureA assay, tested against a tenfold dilution series of H. suis DNA. Cycle threshold (Ct) values are tabulated (above). Amplification plots of a 10-fold dilution series on H. suis DNA samples, containing 2.22 × 107 to 2.22 × 101 bacterial genome equivalents per reaction, are shown (below). The baseline threshold (horizontal red line) is set at 337800 to optimise signal-to-noise.

Article Snippet: The non-Helicobacter panel was made up of the following: (i) 20 species available represented by 21 extracts (Ghent University): Actinobacillus equuli, Aeromonas hydrophila, Bacillus licheniformis, Campylobacter fetus, C. jejuni, C. upsaliensis, Enterococcus faecalis, Escherichia coli, Fusobacterium gastrosuis, Klebsiella pneumoniae, Mannheimia haemolytica, Pasteurella multocida, Proteus vulgaris, Pseudomonas aeruginosa, Salmonella typhimurium, Staphylococcus aureus, Streptococcus equi subsp. Equi, Streptococcus equi subsp. Zooepidemicus, Streptococcus pseudintermedius, Streptococcus suis and Yersinia pseudotuberculosis and (ii) 1 further species (DSMZ): Wolinella succinogenes, an epsilon subclass of Proteobacteria, which is closely related to H. pylori, H. hepaticus and Campylobacter jejuni.

Techniques: Amplification